PMV Connect

Monagle/Ignjatovic
Expertise
We can help with gel-based proteomics (incl. 2D-DIGE) and have the capacity to perform automated spot-picking, with particular experience in analysis of plasma samples.
Contact

A/Prof Vera Ignjatovic
Email: vera.ignjatovic@mcri.edu.au
Phone: 9936 6520

Kemp Laboratory – St Vincent’s Institute of Medical Research
Expertise
We can assist with a range of protein characterisation matters, particularly those related to phosphorylation and other post-translational modifications. We also do cell-signaling metabolite measurements including malonyl-CoA and Di-Methylarginine.
Contact

Prof Bruce Kemp
Email: bkemp@svi.edu.au
Phone: 9288 2480

Hubbard Laboratory – Melbourne University
Expertise
We can provide guidance in protein identification and characterisation, having particular experience with the challenges of microscale samples.
Contact

Dr Mike Hubbard
Email: mike.hubbard@unimelb.edu.au
Phone: 9345 5028

Hill Laboratory – La Trobe University
Expertise
We can help with gel-based proteomics, DIGE and multiplex Westerns, and have complementary experience with RNAi (incl. lentiviral) and exosome-derived biomarker discovery.
Contact

Prof Andrew Hill
Email: andrew.hill@latrobe.edu.au
Phone: 9479 1224

Monash Biomedical Mass Spectrometry Facility – Service Laboratory
Expertise
We can help with protein identification, characterisation and differential quantitation through access to a variety of mass spectrometers, 2D gels (DIGE), Edman sequencing and associated equipment.
Work is accepted from all academic and commercial clients, however, first call on our time will be Monash University and its associated institutions.

Mass spectrometers

Orbitrap Fusion Tribrid (Thermo Scientific)​
2x Q-Exactive Plus Hybrid Quadrupole - Orbitrap (Thermo Scientific)​
Q-Exactive Hybrid Quadrupole - Orbitrap (Thermo Scientific)
2x TripleTOF 6600 (SCIEX)​​
QTrap 6500 (SCIEX)​​​
MicroTOFq (Bruker)​
UltrafleXtreme MALDI TOF/TOF (Bruker)

Off-line HPLC Systems​

​LC 1100 Analytical System (Agilent Technologies)
LC 1200 Series Nano and Capillary (Agilent Technologies)
Ultimate 3000 Nano Systems (Thermo Scientific)

N-terminal sequencers​​

​PPSQ-53A Protein Sequencer (Shimadzu)
2x Procise Protein Sequencer (Applied Biosystems)
Contact

Dr Ralf Schittenhelm
Email: ralf.schittenhelm@monash.edu
Phone: 9902 9932

Dr David Steer
Email: david.steer@monash.edu
Phone: 9902 9323

Bio21 Mass Spectrometry Facility – Service Laboratory
Expertise
We can assist with protein identification and characterisation through access to a broad range of mass spectrometers and associated equipment.
Work is accepted from academic and commercial clients.
At present we have:

2 x Thermo NanoLC/ OrbiTrap Fusion Lumos
2 x Thermo NanoLC/ Q Exactive Plus Mass spectrometer
Thermo NanoLC/ OrbiTRAP ELITE ETD mass spectrometer
Themo Exactive Focus
2 x Agilent 6220 LC/ESI-TOF mass spectrometer
ABSCIEX 5500 NanoLC/QTRAP Mass spectrometer
Bruker Microflex MALDI-TOF Mass spectrometer
Shimadzu UHPLC 8060 QQQ

The laboratory also has a number of general equipment items for use:

Deadbox for proteomic sample preparation
Labconco SpeediVac system
Vertis benchtop freeze dryer

We also have:

Four Agilent HPLC systems for sample preparation.
Contact

Dr Nicholas Williamson
Email: nawill@unimelb.edu.au
Phone: 8344 2206

Ching-Seng Ang
Phone: 8344 2540

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