PMV Connect
Bio21 Mass Spectrometry Facility – Service Laboratory
Expertise
We can assist with protein identification and characterisation through access to a broad range of mass spectrometers and associated equipment.
Work is accepted from academic and commercial clients.
At present we have:
2 x Thermo NanoLC/ OrbiTrap Fusion Lumos
2 x Thermo NanoLC/ Q Exactive Plus Mass spectrometer
Thermo NanoLC/ OrbiTRAP ELITE ETD mass spectrometer
Themo Exactive Focus
2 x Agilent 6220 LC/ESI-TOF mass spectrometer
ABSCIEX 5500 NanoLC/QTRAP Mass spectrometer
Bruker Microflex MALDI-TOF Mass spectrometer
Shimadzu UHPLC 8060 QQQ
The laboratory also has a number of general equipment items for use:
Deadbox for proteomic sample preparation
Labconco SpeediVac system
Vertis benchtop freeze dryer
We also have:
Four Agilent HPLC systems for sample preparation.
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Biomolecular Interaction Facility – CSIRO Parkville
Expertise
We can assist with the application of sensor platform technology to resolve the binding specificity, kinetic parameters and affinity associated with molecular interactions (protein-protein, protein-small molecule).
Work is accepted from academic and commercial clients.
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Hatters Laboratory – Melbourne University
Expertise
We can assist with the design and implementation of fluorescence approaches to investigate protein conformational changes directly in live cells using confocal microscopy. We also investigate protein-protein complexes and heterogeneity using analytical ultracentrifugation.
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Hill Laboratory – La Trobe University
Expertise
We can help with gel-based proteomics, DIGE and multiplex Westerns, and have complementary experience with RNAi (incl. lentiviral) and exosome-derived biomarker discovery.
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Hubbard Laboratory – Melbourne University
Expertise
We can provide guidance in protein identification and characterisation, having particular experience with the challenges of microscale samples.
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Jones Laboratory – RMIT
Expertise
Research in the Jones group specialises in developing and applying metabolomic techniques for environmental research. We can help with the design and running of experiments involving the use and development of NMR spectroscopy, high-resolution, multidimensional chromatographic and mass spectrometric analysis for a range of small molecules (e.g. lipids, amino acids, fatty acids, polyphenols, steroids, pesticides etc) as well as a range of associated bioinformatic techniques.
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Kemp Laboratory – St Vincent’s Institute of Medical Research
Expertise
We can assist with a range of protein characterisation matters, particularly those related to phosphorylation and other post-translational modifications. We also do cell-signaling metabolite measurements including malonyl-CoA and Di-Methylarginine.
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La Trobe University Mass Spectrometry Facility – Service Laboratory
Expertise
This state of the art facility, situated within the La Trobe Institute for Molecular Science (LIMS) offers a comprehensive range of different types of mass spectrometry equipment. Services include qualitative and quantitative mass spectrometry; HPLC sample fractionation, Advion TriVersa Nanomate robot front end, 2D SDS-PAGE and OFFgel electrophoresis. The facility is a member of the Victorian Platform Technologies Network.
Work is accepted primarily from LTU scientists but other academic and commercial clients will be considered as time permits.
Please contact:
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McConville Laboratory – Bio21
Expertise
We can help with many aspects of metabolomics, particularly those based on mass spectrometry.
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Meikle Laboratory – Baker IDI
Expertise
We can help with metabolomics studies, particularly those focused on lipids. Quantification is available for over 200 lipid species (phospholipids, sphingolipids, cholesterols).
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Metabolomics Australia, Victorian Node – Service Laboratory
Expertise
We can assist with metabolite analyses and metabolomics approaches through access to a broad range of mass spectrometers, NMR instruments and associated equipment. Available services: Quantitative targeted metabolite analyses, metabolite profiling, metabolic fingerprinting, biomarker discovery. Method development in collaboration with clients is available.
Work is accepted from academic and commercial clients.
Website
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Monagle/Ignjatovic
Expertise
We can help with gel-based proteomics (incl. 2D-DIGE) and have the capacity to perform automated spot-picking, with particular experience in analysis of plasma samples.
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Monash Antibody Technologies Facility – Monash University
Expertise
We offer custom generation of high-affinity murine monoclonal antibodies with guaranteed IgG isotypes and exceptionally short lead times due to our high-throughput production and screening capabilities. Additional services include peptide support (design, arranging synthesis and coupling), and antibody characterisation and purification. Work is accepted from academic and commercial clients.
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Monash Biomedical Mass Spectrometry Facility – Service Laboratory
Expertise
We can help with protein identification, characterisation and differential quantitation through access to a variety of mass spectrometers, 2D gels (DIGE), Edman sequencing and associated equipment.
Work is accepted from all academic and commercial clients, however, first call on our time will be Monash University and its associated institutions.
Mass spectrometers
Orbitrap Fusion Tribrid (Thermo Scientific)
2x Q-Exactive Plus Hybrid Quadrupole - Orbitrap (Thermo Scientific)
Q-Exactive Hybrid Quadrupole - Orbitrap (Thermo Scientific)
2x TripleTOF 6600 (SCIEX)
QTrap 6500 (SCIEX)
MicroTOFq (Bruker)
UltrafleXtreme MALDI TOF/TOF (Bruker)
Off-line HPLC Systems
LC 1100 Analytical System (Agilent Technologies)
LC 1200 Series Nano and Capillary (Agilent Technologies)
Ultimate 3000 Nano Systems (Thermo Scientific)
N-terminal sequencers
PPSQ-53A Protein Sequencer (Shimadzu)
2x Procise Protein Sequencer (Applied Biosystems)
Contact
Protein Production Unit – Monash University
Expertise
We can help with recombinant protein expression, screening of expression conditions, small and large scale protein purification, and QA. Work is accepted from academic and commercial clients.
Key instrumentation
- AKTA Express units (12 units)
- Liquid handling robot: Tecan Evo
- RT-PCR machine
- AKTA Avant
- Bacterial, yeast and insect/mammalian suspension growth facilities
Contact
WEHI Proteomics Laboratory
Expertise
We can help with protein identification and characterisation through access to a broad range of mass spectrometers and associated equipment. Bioinformatics support is also available.
Work is accepted primarily from WEHI scientists but other academic/commercial clients will be considered as time permits.
Mass spectrometers:
timsTOF (Bruker)
Impact II (Bruker)
MaXis II (Bruker)
Q-Exactive Classic (Thermo Fisher Scientific)
LTQ Orbitrap XL (Thermo Fisher Scientific)
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