PMV Connect

Bio21 Mass Spectrometry Facility – Service Laboratory
Expertise
We can assist with protein identification and characterisation through access to a broad range of mass spectrometers and associated equipment.
Work is accepted from academic and commercial clients.
At present we have:
2 x Thermo NanoLC/ OrbiTrap Fusion Lumos
2 x Thermo NanoLC/ Q Exactive Plus Mass spectrometer
Thermo NanoLC/ OrbiTRAP ELITE ETD mass spectrometer
Themo Exactive Focus
2 x Agilent 6220 LC/ESI-TOF mass spectrometer
ABSCIEX 5500 NanoLC/QTRAP Mass spectrometer
Bruker Microflex MALDI-TOF Mass spectrometer
Shimadzu UHPLC 8060 QQQ
The laboratory also has a number of general equipment items for use:
Deadbox for proteomic sample preparation
Labconco SpeediVac system
Vertis benchtop freeze dryer
We also have:
Four Agilent HPLC systems for sample preparation.
Contact
Biomolecular Interaction Facility – CSIRO Parkville
Expertise
We can assist with the application of sensor platform technology to resolve the binding specificity, kinetic parameters and affinity associated with molecular interactions (protein-protein, protein-small molecule).
Work is accepted from academic and commercial clients.
Contact

Hatters Laboratory – Melbourne University
Expertise
We can assist with the design and implementation of fluorescence approaches to investigate protein conformational changes directly in live cells using confocal microscopy. We also investigate protein-protein complexes and heterogeneity using analytical ultracentrifugation.
Contact

Hill Laboratory – La Trobe University
Expertise
We can help with gel-based proteomics, DIGE and multiplex Westerns, and have complementary experience with RNAi (incl. lentiviral) and exosome-derived biomarker discovery.
Contact

Hubbard Laboratory – Melbourne University
Expertise
We can provide guidance in protein identification and characterisation, having particular experience with the challenges of microscale samples.
Contact

La Trobe University Mass Spectrometry Facility – Service Laboratory
Expertise
This state of the art facility, situated within the La Trobe Institute for Molecular Science (LIMS) offers a comprehensive range of different types of mass spectrometry equipment. Services include qualitative and quantitative mass spectrometry; HPLC sample fractionation, Advion TriVersa Nanomate robot front end, 2D SDS-PAGE and OFFgel electrophoresis. The facility is a member of the Victorian Platform Technologies Network.
Work is accepted primarily from LTU scientists but other academic and commercial clients will be considered as time permits.
Please contact:
Contact

Monagle/Ignjatovic
Expertise
We can help with gel-based proteomics (incl. 2D-DIGE) and have the capacity to perform automated spot-picking, with particular experience in analysis of plasma samples.
Contact

Monash Antibody Technologies Facility – Monash University
Expertise
We offer custom generation of high-affinity murine monoclonal antibodies with guaranteed IgG isotypes and exceptionally short lead times due to our high-throughput production and screening capabilities. Additional services include peptide support (design, arranging synthesis and coupling), and antibody characterisation and purification. Work is accepted from academic and commercial clients.
Contact

Monash Biomedical Mass Spectrometry Facility – Service Laboratory
Expertise
We can help with protein identification, characterisation and differential quantitation through access to a variety of mass spectrometers, 2D gels (DIGE), Edman sequencing and associated equipment.
Work is accepted from all academic and commercial clients, however, first call on our time will be Monash University and its associated institutions.
Mass spectrometers
Orbitrap Fusion Tribrid (Thermo Scientific)
2x Q-Exactive Plus Hybrid Quadrupole - Orbitrap (Thermo Scientific)
Q-Exactive Hybrid Quadrupole - Orbitrap (Thermo Scientific)
2x TripleTOF 6600 (SCIEX)
QTrap 6500 (SCIEX)
MicroTOFq (Bruker)
UltrafleXtreme MALDI TOF/TOF (Bruker)
Off-line HPLC Systems
LC 1100 Analytical System (Agilent Technologies)
LC 1200 Series Nano and Capillary (Agilent Technologies)
Ultimate 3000 Nano Systems (Thermo Scientific)
N-terminal sequencers
PPSQ-53A Protein Sequencer (Shimadzu)
2x Procise Protein Sequencer (Applied Biosystems)
Contact

Protein Production Unit – Monash University
Expertise
We can help with recombinant protein expression, screening of expression conditions, small and large scale protein purification, and QA. Work is accepted from academic and commercial clients.
Key instrumentation
- AKTA Express units (12 units)
- Liquid handling robot: Tecan Evo
- RT-PCR machine
- AKTA Avant
- Bacterial, yeast and insect/mammalian suspension growth facilities
Contact

WEHI Proteomics Laboratory
Expertise
We can help with protein identification and characterisation through access to a broad range of mass spectrometers and associated equipment. Bioinformatics support is also available.
Work is accepted primarily from WEHI scientists but other academic/commercial clients will be considered as time permits.
Mass spectrometers:
timsTOF (Bruker)
Impact II (Bruker)
MaXis II (Bruker)
Q-Exactive Classic (Thermo Fisher Scientific)
LTQ Orbitrap XL (Thermo Fisher Scientific)
Contact
Terms & Conditions
Any information collected for PMV membership will be kept confidential and used only for PMV membership purposes. PMV is a non-profit organisation and will not share any personal or contact details with vendors or other external organisations.
Your membership details are essential for communication purposes. If the news check box is selected, PMV will send occasional emails alerting members to important events and PMV meetings.